Chapter 9
Microfluidic Immunoassays

9.1		Introduction
9.1.1		Microfluidic immunoassay design/operation considerations
9.1.2		Example microfluidic immunoassay formats
9.2		Materials
9.2.1		Microfluidic device
9.2.2		Pumps and interconnections
9.3		Methods
9.3.1		Fabrication of flowcells
9.3.2		Sample and reagent delivery
9.4		Data acquisition and results
9.4.1		FMIA
9.4.2		CGIA
9.5		Discussion
9.5.1		FMIA
9.5.2		CGIA
9.5.3		Challenges of analyzing complex samples
9.6		Acknowledgments, References

Chapter 10
Droplet Based Microfluidics by Shear-Driven Microemulsions

10.1		Introduction
10.1.1		Advantages of droplet-based microfluidics
10.2		Biomedical applications of droplet microfluidics
10.2.1		Bioassays
10.2.2		Particle formation
10.2.3		Therapeutic delivery
10.3		Materials
10.4		Methods
104.1		Channel surface modification
10.4.2		Hydrophilic surface treatment
10.4.3		Hydrophobic surface treatment
10.4.4		Solution preparation
10.4.5		Generation of droplets
10.5		Data acquisition
10.5.1		Droplet arrays
10.5.2		High-speed cameras
10.5.3		Conventional imaging methods
10.6		Discussion and commentary, Acknowledgements, References

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