Chapter 3
Micromixing Within Microfluidic Devices

3.1		Introduction
3.2		Materials
3.2.1		Microfluidic mixing devices
3.2.2		Microfluidic interconnects
3.2.3		Optical assembly
3.2.4		Required reagents
3.3		Experimental design and methods
3.3.1		Passive micromixers
3.3.2		Active micromixers
3.3.3		Multiphase mixers
3.4		Data acquisition, anticipated results, and interpretation
3.4.1		Computer acquisition
3.4.2		Performance metrics, extent of mixing, reaction monitoring
3.5		Discussion and commentary
3.6		Troubleshooting
3.7		Application notes
3.8		Summary points, References

Chapter 4
On-Chip Electrophoresis and Isoelectric Focusing Methods for Quantitative Biology

4.1		Introduction
4.1.1		Microfluidic electrophoresis supports quantitative biology and medicine
4.1.2		Biomedical applications of on-chip electrophoresis
4.2		Materials
4.2.1		Reagents
4.2.2		Facilities/equipment
4.3		Methods
4.3.1		On chip polyacrylamide gel electrophoresis (PAGE)
4.3.2		Polyacrylamide gel electrophoresis based isoelectric focusing
4.3.3		Data acquisition, anticipated results, and interpretation
4.4		Results and discussion
4.5		Discussion of pitfalls
4.6		Summary notes
4.7		Acknowledgments, References

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