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Chapter 13
Microtubule Motors in Microfluidics
| 13.1 |
|
Introduction |
| 13.2 |
Materials |
| 13.2.1 |
Kinesin expression and purification materials |
| 13.2.2 |
Tubulin purification materials |
| 13.2.3 |
Microtubule gliding assay materials |
| 13.2.4 |
Microfabrication materials |
| 13.3 |
Methods |
| 13.3.1 |
Kinesin expression and purification |
| 13.3.2 |
Tubulin purification and labeling |
| 13.3.3 |
Standard protocol for the microtubule gliding assay |
| 13.3.4 |
Design considerations for integrating motor proteins into
microfluidic devices |
| 13.3.5 |
Fabricating enclosed glass channels for microtubule transport |
| 13.4 |
Results |
| 13.5 |
Discussion of pitfalls |
| 13.5.1 |
Kinesin purification |
| 13.5.2 |
Tubulin |
| 13.5.3 |
Motility assays |
| 13.5.4 |
Motility in microchannels |
| 13.5.5 |
Final comments |
| 13.7 |
Acknowledgments, References |
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