Chapter 11
MicroFACS System

11.1		Introduction
11.2		Materials
11.3		Methods
11.4		Results
11.5		Discussion of pitfall
11.6		Statistical analysis
11.7		Application notes
11.8		Summary points
11.9		Acknowledgments, References

Chapter 12
Optical Flow Characterization—Microparticle Image Velocimetry (μPIV)

12.1		Introduction
12.2		Materials and methods
12.2.1		Experimental setup
12.2.2		Volume illumination
12.2.3		Processing algorithms
12.3		Measurement procedures
12.3.1		Step-by-step operations
12.4		Discussion and commentary
12.4.1		Diffraction limit
12.4.2		Particle size effects
12.4.3		Ultimate spatial resolution
12.4.4		Velocity errors
12.4.5		Other flow visualization techniques based on μPIV
12.6		Summary points
12.7		Application notes
12.7.1		Principles of diffusometry
12.7.2		μPIV-based thermometry
12.7.3		Biosensing
12.7.4		Wall shear stress (WSS) measurement
12.8		Future developments, Acknowledgements, References

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