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 |
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Chapter 9
Targeting the Stem Cell Niche In Vivo
| 9.1 |
|
Introduction |
| 9.2 |
Experimental Design |
| 9.3 |
Materials |
| 9.3.1 |
PTH Treatment: |
| 9.3.2 |
Obtaining bone marrow mononuclear cells (BM MNCs): |
| 9.3.3 |
Immunophenotypic enumeration of HSC number |
| 9.3.4 |
Functional enumeration of HSC number |
| 9.4 |
Methods |
| 9.4.1 |
Treatment of mice with PTH |
| 9.4.2 |
Immunophenotypic enumeration of HSC number |
| 9.4.3 |
Functional enumeration of HSC number |
| 9.5 |
Anticipated results |
| 9.6 |
Discussion |
| |
Troubleshooting of Stem Cell Expansion Assays |
| 9.7 |
Application Notes |
| 9.8 |
Summary Points |
|
Chapter 10
Parabiosis in Aging Research and Regenerative Medicine
| 10.1 |
|
Introduction |
| 10.1.1 |
What is Parabiosis? |
| 10.1.2 |
Animal species |
| 10.1.3 |
Physiology of joining |
| 10.1.4 |
History of Parabiosis and the Range of its Biomedical Applications |
| 10.1.5 |
Parabiosis in Aging Studies |
| 10.2 |
Experimental Design for Aging studies |
| 10.3 |
Materials |
| 10.4 |
Methods: |
| 10.4.1 |
Parabiosis Protocol |
| 10.4.2 |
Experimental Method |
| 10.4.3 |
Post operative care |
| 10.4.4 |
Removing the staples |
| 10.4.5 |
Separating a pair |
| 10.5 |
Troubleshooting Parabiotic Disease |
| 10.5.1 |
"Parabiotic Intoxication" |
| 10.5.2 |
Suggestions for the Side-Effects Associated with Parabiotic Disease |
| 10.6 |
Discussion and Summary |
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