| 7.1 |
 |
Introduction |
| 7.2 |
Lineage depletion of whole bone marrow cells |
| 7.2.1 |
Introduction |
| 7.2.2 |
Materials: |
| 7.2.3 |
Common protocols for both Methods |
| 7.2.4 |
Discussion and Commentary |
| |
Troubleshooting Lineage-Depletion Method |
| 7.3 |
Methylcellulose-based in vitro colony-forming assay |
| 7.3.1 |
Introduction |
| 7.3.2 |
Materials |
| 7.3.4 |
Methods |
| 7.3.5 |
Data acquisition, Anticipated results and Interpretation |
| 7.3.6 |
Discussion and Commentary |
| |
Troubleshooting CFU assay |
| 7.4 |
Irradiation of mice for in vivo assays
Troubleshooting Bone Marrow Transplantation |
| 7.5 |
Colony-forming unit spleen (CFU-S) assay |
| 7.5.1 |
Introduction |
| 7.5.2 |
Materials |
| 7.5.3 |
Methods |
| 7.5.4 |
Data acquisition, Anticipated results and Interpretation |
| 7.5.5 |
Discussion and Commentary |
| |
Troubleshooting CFU-S assay |
| 7.6 |
Quantification of HSCs using the limiting dilution assay |
| 7.6.1 |
Introduction |
| 7.6.2 |
Buffers and Materials |
| 7.6.3 |
Methods |
| 7.6.4 |
Data acquisition, Anticipated results and Interpretation |
| 7.6.5 |
Discussion and Commentary |
| |
Troubleshooting Limited Dilution Assay |
| 7.7 |
Summary Notes |
