Chapter 5
Extracellular Matrix Microarrays and Stem Cell Fate

5.1 Introduction 5.2 Experimental Design 5.3 Materials 5.4 Methods 5.4.1 Preparation of substrates for arrays 5.4.2 Fabrication of extracellular matrix arrays 5.4.3 Cell culture on extracellular matrix arrays 5.4.4 Staining, imaging, and data acquisition 5.4.5 Data Analysis 5.5 Anticipated Results 5.6 Discussion and Commentary   Troubleshooting Table 5.7 Application Notes 5.8 Summary Points

Chapter 6
Microfluidic Culture Platform for Investigating the Proliferation and Differentiation of Stem Cells

6.1		Introduction
6.2		Experimental Design
6.2.1		Microfluidic Platform for Differentiation of Human Neural Progenitor Cells
6.2.2		A Hybrid Microfluidic Platform for Stem Cell Biology
6.2.3		Embryonic Stem Cell Response under Dynamically Controlled Gradient Condition
6.3		Materials and Methods
6.3.1		Fabrication of the Microfluidic Device
6.3.2		Fabrication of a Hybrid Microfluidic Platform
6.3.3		Human Neural Stem Cells (hNSCs)
6.3.4		Mouse Neural Stem Cells
6.3.5		Culturing Cells inside the Microfluidic Chamber and Time-lapse Microscopy
6.3.6		Immunocytochemistry
6.4		Data Acquisition, Anticipate Results and Interpretation
6.4.1		Human NSC Proliferation in the Gradient Chamber
6.4.2		Differentiation of Human NSCs into Astrocytes in the Gradient Chamber
6.4.3		Embryonic Stem Cell Response to BMP Signaling
6.5		Discussion and Commentary
		Troubleshooting Table
6.7		Application Notes
6.8		Summary Points

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