Chapter 1
Somatic Cell Nuclear Transfer and Derivation of Embryonic Stem Cells

1.1 Introduction 1.2 Materials for Nuclear Transfer 1.3 Methods for Nuclear Transfer 1.3.1 Preparation of enucleation and nuclear transfer pipettes 1.3.2 Medium preparation 1.3.3 Animal preparation 1.3.4 Nuclear transfer 1.3.5 Enucleation 1.3.6 Preparation of donor cells 1.3.7 Nuclear transfer 1.3.8 Activation 1.3.9 Embryo culture and embryo transfer 1.4 Derivation of mouse ntES cells 1.5 Materials for ES cell Derivation 1.6 Methods for ES cell Derivation 1.6.1 Derivation of ntESCs 1.6.2 In vitro characterization of ntES Cells 1.6.3 In vivo characterization of ntESCs 1.7 Discussion and Commentary   Troubleshooting Table 1.8 Summary points

Chapter 2
Derivation of Mouse Parthenogenetic Embryonic Stem Cells

2.1		Introduction
2.2		Materials
2.2.1		Reagents
2.2.2		Equipment
2.2.3		Media Recipe
2.3		Methods
2.3.1		Generation of p(MI)embryos
2.3.2		Generation of p(MII)embryos
2.3.3		Generation of p(hap)embryos
2.3.4		Derivation of p(MI), p(MII) and p(hap) ES cells
2.3.5		ES cell characterization
2.3.6		Teratoma induction
2.4		Data Acquisition, Anticipated Results and Interpretation
2.5		Discussion and Commentary
		Troubleshooting of Parthenogenetic ES Cell Derivation
2.6		Summary

Previous Chapter | Next Chapter