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Chapter 13
In vivo MR Tracking of hESC-Derived Oligodendrocyte Precursors in Mouse Brain
| 13.1 |
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Introduction |
| 13.2 |
Experimental Design |
| 13.3 |
Materials |
| 13.4 |
Methods |
| 13.4.1 |
Maintenance and differentiation of hES cells |
| 13.4.2 |
Preparation of magnetically labeled cells |
| 13.4.3 |
Confirmation of magnetically labeled cells: histochemical staining |
| 13.4.4 |
Transplantation procedure |
| 13.4.5 |
MRI procedure |
| 13.4.6 |
Data Analysis |
| 13.5 |
Anticipated Results |
| 13.6 |
Discussion and Commentary |
| |
Troubleshooting Table |
| 13.7 |
Application Notes |
| 13.8 |
Summary Points |
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