Chapter 1
Extracellular Matrices for Hepatic Tissue Engineering

1.1 Introduction 1.2 Experimental Design 1.3 Materials 1.4. Methods 1.4.1 Cell Adhesion Assay 1.4.2 Cell Detachment Assay 1.4.3 Inhibition Assay 1.4.4 Western Blot Assay 1.4.5 Flow Cytometry Analysis 1.4.6 Lectin Binding Assay 1.4.7 Coupling of Galactose Groups with ECM Bulk and ECM Surface 1.4.8 Regulation of Density of Galactose and Spatial Orientation of Galactose 1.4.9 Regulation of Topology of ECM 1.4.10 Regulation of Deposition of Natural ECM 1.4.11 Design of Coculture System 1.4.12 Choice of Cell Sources 1.5. Anticipated Results 1.6 Discussion and Commentary 1.7 Application Notes 1.8. Summary Points, Acknowledgments,References

Chapter 2
Tissue Engineering Using a Temperature-Responsive Cell Culture Dish

2.1.		Introduction
2.2.		Experimental Design
2.3		Materials
2.4		Methods
2.4.1		Seeding Cells
2.4.2		Change Medium
2.4.3		Harvest of Cell Sheets
2.4.4		Transplantation of Cell Sheets
2.4.5		Multilayering of Cell Sheets
2.5		Data Acquisition, Anticipated Results, and Interpretation
2.5.1		Autograft/Allograft Models
2.5.2		Xenograft Models
2.6		Discussion and Commentary
2.7		Application Notes
2.7.1		Single Cells
2.7.2		Cell Sheets
2.8		Summary points, Acknowledgments, References

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