Chapter 7
Nanoparticles as Biodynamic Substrates for Engineering Cell Fates

7.1 Introduction 7.2 Experimental Design 7.3 Materials 7.3.1 Cell Culture, Fixing, Staining and Analysis Reagents 7.3.2 Nanoparticle Fabrication and Functionalization 7.3.3 Microscale Plasma Initiated Patterning 7.4 Methods 7.4.1 Albumin Nanoparticle Fabrication 7.4.2 Albumin Nanoparticle Functionalization 7.4.3 Albumin Nanoparticle Pattern Creation – Microscale Plasma Initiated Patterning (μPIP) 7.4.4 Cell Culture 7.4.5 Keratinocyte Morphology and Migration 7.4.6 Fibroblast Extracellular Matrix Assembly 7.4.7 Cell Attachment Assay 7.5 Results 7.5.1 Enhanced Cell Migration 7.5.2 Enhanced Extracellular Matrix Assembly 7.6 Discussion of Pitfalls 7.6.1 Spatial Guidance of Cell Attachment – microscale Plasma Initiated Patterning 7.6.2 Three Dimensional Presentation of Albumin Nanoparticles 7.7 Summary Notes   Acknowledgements   References

Chapter 8
Magnetic cell separation to enrich for rare cells

8.1		Introduction
8.1.1		Principle
8.1.1.1		Magnetic force on an immuno-magnetically labeled cell
8.1.1.2		Interaction between magnetic particles and the targeted cell
8.1.1.3		Quantification of magnetic cell separation performance
8.1.2		Examples of cell magnetic separation applications
8.1.2.1		Isolation of human stem cells
8.1.2.2		T cell depletion
8.1.2.3		Rare cancer cell detection
8.1.2.4		Bacteria
8.2		Materials and Methods
8.2.1		Enrichment process
8.2.2		Red cell lysis step
8.2.3		Immunomagnetic labeling
8.2.4		Magnetic cell separation step
8.3		Data Acquisition, Results and Interpretation
8.4		Discussion and commentary
8.5		Summary points to obtain high performance, magnetic cells separations
		Acknowledgement
		References

Previous Chapter | Next Chapter