Chapter 5
Tracking Single Biomolecules in Live Cells using Quantum Dot Nanoparticles

5.1 Introduction 5.2 Materials 5.2.1 Reagents 5.2.2 Imaging Equipment 5.3 Methods 5.3.1 Forming QD Bioconjugates 5.3.2 Treating cells with QD bioconjugates 5.4 Data Acquisition, Anticipated Results, and Interpretation 5.4.1 Imaging QD-bound complexes in cells 5.4.2 Analysis of the Real Time QD Dynamics 5.5 Discussion and Commentary 5.6 Troubleshooting Table   References

Chapter 6
Designer Self-assembling Peptides Scaffolds for 3-dimensional Tissue Cell Cultures

6.1		Introduction
6.1.1		Discovery and development of self-assembling peptide scaffold
6.1.2		The nanofiber structure of the peptide scaffold
6.1.3		A generic biological scaffold
6.1.4		Peptide scaffold fosters chondrocyte extracellular matrix production
6.1.5		Designer peptides appended with active motifs
6.2		Materials
6.3		Reagents
6.4		Methods
6.4.1		Peptide solution preparation
6.4.2		Designer peptide synthesis and scaffold preparation
6.4.3		Culture cells in plate inserts
6.4.4		Cell culture system
6.4.5		Neural cell culture and seeding
6.4.6		Preparation of MC3T3-E1 cells
6.4.7		Cell culture of human umbilical vein endothelial cells (HUVECs)
6.4.8		Cell proliferation assay
6.4.9		DNA content measurement
6.4.10		Boundary-sandwiched cell migration assay
6.4.11		Fluorescence microscopy
6.4.12		Immunocytochemistry
6.4.13		SEM sample preparation
6.4.14		Circular Dichroism (CD)
6.4.15		Structural study using atomic force microscopy (AFM)
6.4.16		Biomechanical study using rheology
6.4.17		Alkaline Phosphatase (ALP) staining for MC3T3-E1 cells
6.4.18		Biochemical assays for alkaline phosphatase (ALP) activity for MC3T3-E1 cells
6.4.19		Low protein release from the peptide scaffold
6.5		Data Acquisition, Results and Interpretation
6.5.1		Designer self-assembling peptide nanofiber hydrogel scaffold
6.5.2		3D cell cultures
6.5.3		Cell Migration in Peptide Scaffolds
6.5.4		Rheology of Peptide Hydrogel Scaffold
6.5.5		Tissue regeneration and tissue engineering
6.5.6		Protein releases from the peptide nanofiber hydrogel scaffold
6.6		Discussions and Commentary
6.7		Troubleshooting Table
6.8		Applications Notes
6.8.1		In vivo injectable self-assembling peptides
6.8.2		In vitro multi-cell system for tissue engineering
6.8.3		Mixed peptide hydrogel with polymer composites
6.9		Summary Points
		Acknowledgements
		Annotated References
		Supplementary Electronic Materials and Resources

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