Chapter 3
Nanoparticle-Enzyme Hybrids as Bioactive Materials

3.1 Introduction 3.2 Materials 3.3 Methods 3.3.1 Enzyme-Attached Polystyrene Nanoparticles 3.3.2 Polyacrylamide Hydrogel Nanoparticles For Entrapment Of Enzymes 3.3.3 Magnetic Nanoparticles With Porous Silica Coating For Enzyme Attachment 3.3.4 Enzyme Loading And Activity Assay 3.4 Results 3.4.1 Polystyrene-Enzyme Hybrid Nanoparticles 3.4.2 Polyacrylamide Hydrogel Nanoparticles With Entrapped Enzymes 3.4.3 Magnetic Nanoparticles For Enzyme Attachment 3.5 Discussion And Commentary 3.6 Trouble Shooting 3.7 Application Notes 3.8 Summary And Conclusions   Acknowledgments   References

Chapter 4
Self-Assembled QD-Protein Bioconjugates and their Use in Fluorescence Resonance Energy Transfer

4.1		Introduction
4.2		Materials
4.2.1		Reagents
4.2.2		Equipment
4.3		Examples of Data-Driven Modeling
4.3.1		Quantum Dot Synthesis
4.3.2		Surface Ligand Exchange
4.3.2.1		DHLA Preparation
4.3.2.2		Ligand Exchange and Transfer Of Hydrophobic Cdse-Zns Qds Into Water
4.3.3		Biomolecule Conjugation
4.3.3.1		Biomolecule Conjugation Protocol
4.3.4		Fluorescence Measurements
4.3.4.1		Fluorescence Spectra Acquisition Protocol
4.4		Data Analysis And Interpretation
4.4.1		Calculating Donor-Acceptor Distances
4.4.2		Calculating reaction rates of surface-bound substrates
4.5		Summary Points
4.6		Conclusions
		Annotated References

Previous Chapter | Next Chapter