Chapter 11
Biomedical Applications Of Metal Nanoshells

11.1 Introduction 11.1.1 Biomedical Applications of Metal Nanoshells 11.1.2 Nanoshells for Combined Optical Contrast & Therapeutic Application 11.2 Experimental Design 11.3 Materials 11.3.1 Nanoparticle Production 11.3.2 Protein Conjugation to Nanoshells Surface 11.3.3 Cell Culture 11.3.4 In Vitro Assays 11.4 Methods 11.4.1 Fabrication of Gold / Silica Core Nanoshells 11.4.2 Nanoshells for Combined Imaging and Therapy In Vivo 11.4.2.1 In Vivo Model 11.4.2.2 Oct Imaging 11.4.2.3 Therapeutic Laser Irradiation 11.4.2.4 Nanoshell Accumulation in Tissue 11.4.3 Passivation of Nanoshells with Peg 11.4.4 Conjugation of Bio-Molecules to Nanoshells 11.4.5 Quantification of Antibodies on Nanoshells 11.5 Results 11.5.1 Gold / Silica Nanoshells Allow Both Imaging Contrast Increase And Therapeutic Benefit 11.5.1.1 Oct Image Analysis 11.5.1.2 Histological Analysis 11.5.1.3 Survival Following Imaging and Therapy 11.5.2 Evaluation of Antibody Concentration Per Nanoshell 11.6 Discussion of Pitfalls 11.7 Statistical Analysis 11.8 Troubleshooting Table 11.9 References

Chapter 12
Environmentally-Responsive Multifunctional Liposomes

12.1		Introduction: Biological Networks And Reverse Engineering
12.2		Materials
12.2.1		Chemicals
12.2.2		Syntheses
12.2.3		Preparation of the TATp-Bearing, Rhodamine-Labeled Liposomal Formulations
12.2.4		Preparation of the TATp-Bearing, Rhodamine Labeled, pGFP Complexed Liposomal Formulations
12.3		Methods
12.3.1		Synthesis of Hydrazone-Based mPEG-HZ-PE Conjugates
12.3.1.1		Synthesis of Aliphatic Aldehyde-Derived Hydrazone-Based mPEG-HZ-PE Conjugates
12.3.1.2		Synthesis of Aromatic Aldehyde-Derived Hydrazone-Based mPEG-HZ-PE Conjugates
12.3.1.3		Synthesis of Aromatic Ketone-Derived Hydrazone-Based mPEG-HZ-PE Conjugates
12.3.2		Synthesis of PE-PEG1000-TATp Conjugate
12.3.3		in Vitro Ph-Dependant Degradation of PEG-HZ-PE Conjugates
12.3.4		Avidin-Biotin Affinity Chromatography
12.3.5		in Vitro Cell-Culture Study
12.3.6		in Vivo Study
12.3.7		in Vivo Transfection with PGFP
12.4		Discussion and Commentary
12.4.1		Synthesis of Hydrazone-Based mPEG-HZ-PE Conjugates
12.4.2		Synthesis of PE-PEG1000-TATp Conjugate
12.4.3		in Vitro Ph-Dependant Degradation of PEG-HZ-PE Conjugates
12.4.4		Avidin-Biotin Affinity Chromatography
12.4.5		in Vitro Cell Culture Study
12.4.6		in Vivo Study
12.4.7		in Vivo pGFP Transfection Experiment
12.5		Conclusion
12.6		Troubleshooting
12.7		Summary Points
12.8		Acknowledgements
		References

Previous Chapter | Next Chapter