Chapter 7
Image-Based Cell Sorting using Microscale Electrical and Optical Actuation

7.1 Introduction 7.1.1 Electrical and optical microscale cell manipulation 7.2 Materials 7.2.1 Materials for Microfabrication 7.2.2 Cell lines and culture 7.2.3 Buffers and reagents 7.2.4 Staining 7.2.5 Equipment 7.3 Methods 7.3.1 Material choices and fabrication 7.3.2 Packaging and Experimental Setup 7.4 Data Acquisition, Anticipated Results and Interpretation 7.4.1 Cell Culture and Assay 7.4.2 Imaging and Sorting 7.5 Discussion and Commentary 7.6 Troubleshooting Table 7.7 Summary Points

Chapter 8
Pharmacokinetic-Pharmacodynamic Models on a Chip

8.1		Introduction
8.2		Pharmacokinetic-Pharmacodynamic modeling
8.2.1		Basic concept
8.2.2		Pharmacokinetic model
8.2.2.1		Classical PK model
8.2.2.2		PBPK model
8.2.3		Pharmacodynamic model
8.2.3.1		Simple direct effects
8.2.3.2		Irreversible effects
8.2.3.3		Tolerance model
8.2.4		Integrated PK-PD modeling
8.3		Micro Cell Culture Analog (μCCA)
8.3.1		Design of a µCCA and calculation of flow rates
8.3.2		Fabrication of a µCCA
8.3.2.1		Materials
8.3.2.2		Methods
8.3.3		Cell seeding and assembly of the device
8.3.3.1		Materials
8.3.3.2		Methods
8.3.4		Data Acquisition, Anticipated results, and Interpretation
8.3.5		Discussion and Commentary
8.3.5.1		Operation of a µCCA
8.3.5.2		Authenticity of cells
8.3.5.3		Noninvasive detection
8.3.6		Troubleshooting
8.3.7		Application notes
8.4		Summary points

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