Chapter 5
A Microfluidic Tool for Immobilizing C. Elegans

5.1 Introduction 5.2 Materials 5.3 Methods 5.4.1 Overview and timeline 5.4.2 Designing the device and ordering the photomask 5.4.3 Fabricating the master for the device 5.4.4 Replica molding the master in PDMS 5.4.5 Preparing C. elegans for loading 5.4.6 Assembly of the microfluidic device 5.4.7 Preparing the device for loading 5.4.8 Loading worms into the device 5.4.9 Unloading worms from the device 5.5 Data Acquisition, Anticipated Results and Interpretation 5.6 Discussion and Commentary 5.7 Troubleshooting Table 5.8 Application Notes 5.9 Summary Points

Chapter 6
Osmolality Control for Microfluidic Embryo Cell Culture using Hybrid poly(dimethylsiloxane) (PDMS)-Parylene Membranes

6.1		Introduction
6.2		Experimental Design
6.3		Materials
6.3.1		Reagents
6.3.2		Equipment
6.4		Methods
6.4.1		PDMS-parylene-PDMS Membrane Preparation
6.4.2		Preparation of Glass Slides and Bonding to Hybrid Membranes
6.4.3		Embryo preparation
6.5		Troubleshooting
6.6		Data Acquisition/Anticipated Results/Interpretation
6.7		Discussion and Commentary
6.8		Application Notes
6.9		Summary Points

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