Chapter 1
Immunoaffinity Capture of Cells from Whole Blood

1.1 Introduction 1.2 Experimental Design 1.3 Materials 1.4 Methods 1.4.1 Device Fabrication 1.4.2 Surface modification 1.4.3 Cell capture 1.4.4 Post-capture processing 1.5 Data Acquisition, anticipated results, and interpretation 1.6 Discussion and commentary 1.7 Troubleshooting table 1.8 Application notes 1.9 Summary Points

Chapter 2
Dynamic Gene Expression Analysis in a Living Cell Array

2.1		Introduction
2.2		Materials
2.3		Methods
2.3.1		GFP Reporter Cell Line Construction
2.3.2		Microfluidic Cell Array Fabrication
2.3.3		Microfluidic Array Pretreatment and Seeding
2.3.4		Stimulation and Reporter Imaging
2.4		Data Acquisition, Anticipated Results And Interpretation
2.5		Discussion
2.6		Troubleshooting Table
2.7		Application Notes
2.8		Summary Points

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