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Chapter 11
Subtractive Methods for Forming Microfluidic Gels of Extracellular Matrix Proteins
| 11.3 |
|
Introduction |
| 11.4 |
Materials |
| 11.4.1 |
Supporting dishes |
| 11.4.2 |
PDMS housing |
| 11.4.3 |
Removable elements (needles & gelatin mesh) |
| 11.4.4 |
ECM proteins |
| 11.4.5 |
High-flow perfusion |
| 11.5 |
Methods |
| 11.5.1 |
Construction of supporting dishes |
| 11.5.2 |
Construction of PDMS housings |
| 11.5.3 |
Preparation of removable elements |
| 11.5.4 |
Formation of microfluidic gels |
| 11.5.5 |
Perfusion of microfluidic gels |
| 11.6 |
Anticipated Results |
| 11.7 |
Application Notes |
| 11.7.1 |
Rate of gelation |
| 11.7.2 |
Resistance of microfluidic gels and tubing |
| 11.8 |
Discussion and Commentary |
| 11.8.1 |
Enlarged and/or deformed channels |
| 11.8.2 |
Leaks between the gel and PDMS or between the gel and coverslip |
| 11.8.3 |
Troubleshooting Table |
| 11.9 |
Summary Points |
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