Chapter 5
A Liposome Assay for Evaluating the Ocular Toxicity of Chemicals

5.1 Introduction 5.2 Experimental Design 5.3 Materials 5.4 Methods 5.4.1 Preparation of calcein-loaded liposomes 5.4.2 Separation of bulk calcein from loaded liposomes with Sephadex 5.4.3 Ocular toxicity experiments using dye-loaded liposomes 5.5 Data Acquisition, Anticipated Results, and Interpretation 5.6 Discussion and Commentary 5.7 Application Notes 5.8 Summary Points, References

Chapter 6
Prediction of Potential Drug Myelotoxicity by In Vitro Assays on Hematopoietic Progenitors

6.1		Introduction
6.2		Experimental Design
6.3		Materials
6.3.1		Reagents
6.4		Methods
6.4.1		Preparation of methylcellulose stocks
6.4.2		Source of murine hematopoietic progenitors
6.4.3		Source of human hematopoietic progenitors
6.4.4		Technical procedure for GM-CFU test
6.4.5		Passing from screening phase to IC determination phase
6.4.6		Incubator humidity test
6.4.7		Scoring the colonies
6.4.8		Criteria for colony counting
6.5		Data Acquisition, Anticipated Results, and Interpretation
6.5.1		Statistical guidelines
6.6		Discussion and Commentary
6.7		Application Notes
6.8		Summary Points, Acknowledgments, References

Previous Chapter | Next Chapter