|
 |
|
Chapter 3
Aggregating Brain Cell Cultures for Neurotoxicity Tests
| 3.1 |
|
Introduction |
| 3.2 |
Experimental Design |
| 3.3 |
Materials |
| 3.3.1 |
Animals |
| 3.3.2 |
Special equipment |
| 3.3.3 |
Reagents |
| 3.3.4 |
Preparation of solutions and media |
| 3.4 |
Methods |
| 3.4.1 |
Washing and sterilizing the glassware |
| 3.4.2 |
Cell isolation and culture preparation |
| 3.4.3 |
Maintenance of aggregating brain cell cultures (media
replenishment and subdivision) |
| 3.4.4 |
Preparation and treatment of replicate cultures |
| 3.4.5 |
Harvest of replicate cultures for various analytical procedures |
| 3.4.6 |
Examples of sample preparation and use for various analytical
procedures |
| 3.4.7 |
Data Analysis |
| 3.5 |
Anticipated Results |
| 3.6 |
Discussion and Commentary |
| 3.7 |
Application Notes |
| 3.8 |
Summary Points, Acknowledgments, References |
|
Chapter 4
Approaches Towards a Multiscale Model of Systemic Inflammation in Humans
| 4.1 |
|
Introduction |
| 4.2 |
Materials |
| 4.2.1 |
Human endotoxin model and data collection |
| 4.3 |
Methods |
| 4.3.1 |
Transcriptional dynamics and intrinsic responses |
| 4.3.2 |
Modeling inflammation at the cellular level |
| 4.3.3 |
Modeling inflammation at the systemic level |
| 4.4 |
Results |
| 4.4.1 |
Elements of the multiscale host response model of human
inflammation |
| 4.4.2 |
Estimation of relevant model parameters |
| 4.4.3 |
Qualitative assessment of the model |
| 4.5 |
Conclusions, Acknowledgments, References |
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