Chapter 1
Current Methods for Prediction of Human Hepatic Clearance Using In Vitro Intrinsic Clearance

1.1 Introduction 1.2 Materials 1.3 Methods 1.3.1 Thawing the hepatocytes 1.3.2 Clearance study using a hepatocyte suspension 1.3.3 Clearance study using a plated hepatocyte culture 1.3.4 Clearance study using a plated hepatocyte culture under a flow condition 1.3.5 Sampling for the clearance study 1.3.6 Sample analysis using LC-MS/MS 1.4 Data Acquisition, Anticipated Results, and Interpretation 1.4.1 Hepatocyte suspension and plated hepatocyte system 1.4.2 Physiologically based microfluidic systems 1.5 Discussion and Commentary 1.5.1 Hepatocyte suspension system 1.5.2 Plated hepatocyte system 1.5.3 Physiologically based microfluidic systems 1.6 Summary, References

Chapter 2
Use of Permeability from Cultured Cell Lines and PAMPA System and Absorption from Experimental Animals for the Prediction of Absorption in Humans

2.1		Introduction
2.2		Materials
2.3		Methods
2.3.1		Cultured cell system
2.3.2		PAMPA system
2.3.3		In vivo absorption measurements
2.4		Data Acquisition, Anticipated Results, and Interpretation
2.4.1		Data analysis
2.4.2		Results and interpretation
2.5		Discussion and Commentary
2.5.1		Cell culture and PAMPA systems
2.5.2		Absorption in experimental animals
2.5.3		Rats
2.5.4		Dogs
2.5.5		Monkeys
2.6		Summary, References

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