Chapter 13
Alternatives for Absorption Testing

13.1 Introduction 13.2 Materials 13.2.1 Franz diffusion cell 13.2.2 Consumables 13.2.3 Chemicals and solutions 13.2.4 Technical equipment 13.3 Methods 13.3.1 Skin preparation 13.3.2 Determination of skin penetration using the Franz cell setup 13.3.3 Determination of skin permeation using the Franz cell setup 13.3.4 Skin absorption studies with commercially available 3D skin models 13.3.5 Quality control 13.3.6 Data evaluation 13.3.7 Biostatistics 13.4 Results and Discussion 13.5 Discussion of Pitfalls and Troubleshooting 13.6 Summary, References

Chapter 14
A 3D Model of the Human Epithelial Airway Barrier

14.1		Introduction
14.2		Experimental Design
14.3		Materials
14.3.1		General materials
14.3.2		Epithelial cell cultures—thawing
14.3.3		Epithelial cell cultures—culturing
14.3.4		Isolation of monocyte-derived macrophages (MDM) and dendritic cells (MDDC)
14.3.5		Triple cell coculture
14.3.6		Transepithelial electrical resistance (TEER) measurements
14.3.7		Staining for laser scanning microscopy (LSM)
14.3.8		Embedding for transmission electron microscopy (TEM)
14.4		Methods
14.4.1		Epithelial cells
14.4.2		Isolation of monocyte-derived macrophages (MDM) and dendritic cells (MDDC)
14.4.3		TEER measurements
14.4.4		Staining for LSM
14.4.5		Fixation and embedding of cells for transmission electron microscopy (TEM)
14.5		Anticipated Results
14.6		Discussion and Commentary
14.7		Application Notes
14.8		Summary Points, Acknowledgments, References

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