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Chapter 7
Self-Assembly of Cell-Laden Hydrogels on the Liquid-Air Interface
| 7.1 |
|
Introduction |
| 7.2 |
Experimental Design |
| 7.3 |
Materials |
| 7.4 |
Methods |
| 7.4.1 |
Preparation of 1m of 20% poly (ethylene glycol)
diacrylate prepolymer |
| 7.4.2 |
Cell preparation (NIH 3T3) |
| 7.4.3 |
Storage of PEG 125 |
| 7.4.4 |
Mixing PEG solution with cells |
| 7.4.5 |
Glass OTS treatment |
| 7.4.6 |
Photolithography |
| 7.4.7 |
Aggregation process |
| 7.4.8 |
Data analysis |
| 7.5 |
Anticipated Results |
| 7.6 |
Discussion and Commentary |
| 7.7 |
Application Notes |
| 7.8 |
Summary Points, Acknowledgments, References |
|
Chapter 8
3D Encapsulation of Cells in Hydrogels Using Radical and Addition Cross-Linking
| 8.1 |
|
Introduction |
| 8.2 |
Experimental Design |
| 8.3 |
Materials |
| 8.4 |
Methods |
| 8.4.1 |
Preparation of materials |
| 8.4.2 |
Initiation of pendant addition reactions and preparation of
cells for encapsulation |
| 8.4.3 |
Synthesis of hydrogels |
| 8.4.4 |
Characterization of hydrogels |
| 8.4.5 |
Characterization of cellular behavior in gels |
| 8.5 |
Anticipated Results |
| 8.6 |
Discussion and Commentary |
| 8.7 |
Application Notes |
| 8.8 |
Summary Points, References |
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