Chapter 5
Chip-Based Tissue Engineering in Microbioreactors

5.1 Introduction 5.2 Experimental Design 5.3 Materials and Equipment 5.3.1 Fabrication of p- and f-chips 5.3.2 Fabrication of the r-chip 5.3.3 Bioreactor assembly 5.4 Methods 5.4.1 KITChip fabrication (p- and f-chip) 5.4.2 r-chip (SMART technology) 5.4.3 Preparation of KITChips and bioreactors for cell culture 5.4.4 Cell culture 5.4.5 Data analysis 5.5 Anticipated Results 5.6 Discussion and Commentary 5.7 Application Notes 5.8 Summary Points, Acknowledgments, References

Chapter 6
Affinity-Binding Alginate Scaffolds for the Controlled Delivery of Multiple Heparin-Binding Proteins

6.1		Introduction
6.2		Experimental Design
6.3		Materials
6.3.1		Materials for alginate sulfation
6.3.2		Materials for scaffold fabrication and factor loading
6.3.3		Materials for immunohistochemistry
6.4		Methods
6.4.1		General procedure
6.4.2		Preparation of alginate-sulfate and characterization of product
6.4.3		Scaffold fabrication
6.4.4		Triple factor loading into scaffolds and release studies
6.4.5		In vivo studies: immunostaining, imaging, and data acquisition
6.5		Anticipated Results
6.6		Discussion and Commentary
6.7		Application Notes
6.8		Summary Points, Acknowledgments, References

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