| 4.1. |
 |
Introduction |
| 4.1.1 |
Discovery and development of self-assembling
peptide scaffolds |
| 4.1.2 |
The nanofiber structure of the peptide scaffold |
| 4.1.3 |
A generic biological scaffold |
| 4.1.4 |
Peptide scaffold fosters chondrocyte extracellular
matrix production |
| 4.1.5 |
Designer peptides appended with active motifs |
| 4.2 |
Materials |
| 4.3 |
Reagents |
| 4.4 |
Methods |
| 4.4.1 |
Peptide solution preparation |
| 4.4.2 |
Designer peptide synthesis and scaffold preparation |
| 4.4.3 |
Culture cells in plate inserts |
| 4.4.4 |
Cell culture system |
| 4.4.5 |
Neural cell culture and seeding |
| 4.4.6 |
Preparation of MC3T3-E1 cells |
| 4.4.7 |
Cell culture of human umbilical vein endothelial cells (HUVECs) |
| 4.4.8 |
Cell proliferation assay |
| 4.4.9 |
DNA content measurement |
| 4.4.10 |
Boundary-sandwiched cell migration assay |
| 4.4.11 |
Fluorescence microscopy |
| 4.4.12 |
Immunocytochemistry |
| 4.4.13 |
SEM sample preparation |
| 4.4.14 |
Circular dichroism (CD) |
| 4.4.15 |
Structural study using atomic force microscopy (AFM) |
| 4.4.16 |
Biomechanical study using rheology |
| 4.4.17 |
Alkaline phosphatase (ALP) staining for MC3T3-E1 cells |
| 4.4.18 |
Biochemical assays for alkaline phosphatase (ALP)
activity for MC3T3-E1 cells |
| 4.4.19 |
Low protein release from the peptide scaffold |
| 4.5 |
Data Acquisition, Results, and Interpretation |
| 4.5.1 |
Designer self-assembling peptide nanofiber hydrogel scaffold |
| 4.5.2 |
3D cell cultures |
| 4.5.3 |
Cell migration in peptide scaffolds |
| 4.5.4 |
Rheology of peptide hydrogel scaffold |
| 4.5.5 |
Tissue regeneration and tissue engineering |
| 4.5.6 |
Protein releases from the peptide nanofiber hydrogel scaffold |
| 4.6 |
Discussions and Commentary |
| 4.7 |
Application Notes |
| 4.7.1 |
In vivo injectable self-assembling peptides |
| 4.7.2 |
In vitro multicell system for tissue engineering |
| 4.7.3 |
Mixed peptide hydrogel with polymer composites |
| 4.8 |
Summary Points, Acknowledgments, References |
