Chapter 1
Chemical Modification of Porous Scaffolds Using Plasma Polymers

1.1 Introduction 1.2 Experimental Design 1.3 Materials 1.4. Methods 1.4.1 Scaffold preparation 1.4.2 Deposition of plasma polymers 1.4.3 Surface analysis 1.4.4 Cell culture on scaffolds 1.4.5 Alamar Blue assay 1.4.6 Cell viability assay on scaffolds 1.4.7 Scanning electron microscopy 1.4.8 Microcomputed tomography of scaffolds 1.5. Data Acquisition, Anticipated Results, and Interpretation 1.5.1 Surface analysis 1.5.2 Investigation of cell culture on modified scaffolds 1.6. Discussion and Commentary 1.7. Application Notes 1.8. Summary Points, Acknowledgments,References

Chapter 2
Three-Dimensional Cultures in Soft Self-Assembling Nanofibers

2.1.		Introduction
2.2.		Experimental Design
2.3		Materials
2.3.1		Reagents
2.3.2		Equipment
2.4		Methods
2.4.1		Self-assembling peptide preparation
2.4.2		Cell encapsulation into the self-assembling peptide
2.4.3		Sandwich method
2.4.4		Cell isolation and culture of isolated cells
2.4.5		Cryosections of the 3D cultures
2.4.6		Cell proliferation study using 5-bromodeoxyuridine (BrdU) uptake analysis
2.4.7		Cell viability
2.4.8		Protein analysis
2.4.9		Sample staining
2.4.10		sGAG quantification
2.4.11		Lysis of 3D cultures for RNA extraction
2.5		Data Acquisition, Anticipated Results, and Interpretation
2.6		Discussion and Commentary
2.7		Troubleshooting
2.8		Application Notes
2.9		Summary points, Acknowledgments, References

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