Chapter 11
Three-Dimensional Neuronal Cultures

11.1 Introduction 11.1.1 2D versus 3D culture models 11.1.2 Cell type and culture configurations 11.2 Experimental Design 11.3 Materials 11.3.1 Harvest 11.3.2 Dissociation 11.3.3 Plating 11.3.4 Assessment 11.4 Methods 11.4.1 Harvest 11.4.2 Dissociation 11.4.3 Plating 11.4.4 General assessment: staining, imaging, and data acquisition 11.4.5 Data analysis 11.5 Anticipated Results and Discussion 11.5.1 Characterization of cell morphology and viability in 3D neuronal cultures 11.5.2 Cell considerations 11.5.3 Scaffold considerations 11.6 Application Notes and Commentary 11.7 Summary Points, Acknowledgments, References

Chapter 12
Engineering Cartilage Tissue with Zonal Properties

12.1		Introduction
12.2		Materials
12.2.1		Reagents/supplies
12.2.2		Facilities/equipment
12.3		Methods
12.3.1		Obtaining and tracking zonal chondrocytes
12.3.2		Zonal construct formation
12.3.3		Analyses
12.4		Discussion and Commentary
12.5		Summary Points, References

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